Lab on a Bead

Lab on a Bead is a Swedish Biotech company that can provide the next generation high capacity magnetic separation platform for purification of biomolecules. The LOABeads magnetic beads combine the high binding capacity of agarose with fast magnetic separation. LOABeads are a flexible alternative for any bioseparation application, from microliter scale to liter scale.

Advantages of LOABeads
• Superior Binding Capacity
The high surface area of porous magnetic agarose beads results in high binding capacities.

• Fast Magnetic Separation
The LOABeads beads show excellent super-paramagnetic behavior and are strongly attracted to external magnets which enables separation within seconds.

• High Specificity
The hydrophilicity of the agarose offers minimal unspecific binding resulting in highly specific purifications.

PRODUCTS

LOABeads™ Protein A-

These are intended for purification of antibodies from serum and ascites fluid samples, IgG serum depletion and immunoprecipitation. Combined with LOABeads MagSep separators, purification can be performed in standard 15 and 50ml centrifuge tubes, as well as 500ml bottles.

Product Data

Product name	LOABeads™ Protein A
Product number	1001
Particle size	45-165 µm
Ligand	Recombinant protein A
Type magnetization	Super-paramagnetic
Matrix	4% agarose
Product form	10% bead suspension in PBS with 20% ethanol
Binding capacity*	4 mg rabbit IgG/ml bead suspension
Max. binding capacity**	6 mg human IgG/ml bead suspension
Binding buffer	PBS
Elution buffer	60 mM citrate pH 3.0
Storage conditions	+2 to +8°C in PBS with 20% ethanol
Stability information***	24 months
Regeneration	Can be reused multiple times

* 90% binding of rabbit IgG (2 mg/ml) after 60 min reaction.
** Determined in an overloading test at 5 mg IgG/ml after 60 min reaction.
*** Data of product stability is continuously updated based on ongoing stability studies.

LOABeads™ Affiamino-

LOABeads™ AffiAmino are surface activated magnetic agarose beads employing Lab on a Bead’s patented coupling chemistry. Any amino containing ligand, e.g. proteins and antibodies can be covalently bound to the beads using a simple process.

Product Data

Product name	LOABeads™ AffiAmino
Product number	1003
Particle size	45-165 µm
Coupling to	Primary amino groups
Type magnetization	Super-paramagnetic
Matrix	4% agarose
Product form	10% bead suspension in PBS with 20% ethanol
Coupling capacity*	0.5-1 mg IgG/ml bead suspension 5-10 mg IgG/ml settled beads
Binding buffer	PBS with 0.1% tween
Storage conditions	+2 to +8°C in PBS with 20% ethanol
Stability information**	12 months

* 90% coupling of rabbit IgG (1 mg/ml) after 60 min incubation.
** Data of product stability is continuously updated based on ongoing stability studies.

LOABeads MabBind A-

These are magnetic agarose beads coupled with protein A optimized for efficient purification of IgG from cell cultures. The high specificity and high capacity of LOABeads MabBind A ensures that a very pure antibody is obtained with a high yield, even when antibody capture is performed directly in the cell culture medium.

LOABeads MabBind A are ideal to use in many applications of magnetic bead affinity chromatography (MBAC). For example in lab-scale purification of antibodies to be used in R&D and diagnostics and for characterizations of candidate biopharmaceutical IgG antibodies

Product Data

Product name	LOABeads™ MabBind A
Product number	1004
Particle size	45-165 µm
Ligand	Recombinant protein A
Type magnetization	Super-paramagnetic
Matrix	4% agarose
Product form	10% bead suspension in PBS with 20% ethanol
Binding capacity*	3 mg rabbit IgG/ml bead suspension
Binding conditions	Directly in cell culture media, pH 6–8
Elution buffer	60 mM citrate pH 3.0
Storage conditions	+2 to +8°C in PBS with 20% ethanol
Stability information**	12 months
Regeneration	Can be reused multiple times

* Binding capacity was determined by incubating 500µl 10% LOABeads MabBind A (50 µl beads) with rabbit IgG (˜2 mg/ml in 1 ml PBS) for 60 minutes at room temperature. Binding capacity is obtained with a yield of 90% or more under the conditions specified above.
** Data of product stability is continuously updated based on ongoing stability studies.

LOABeads™ MagSep-

These are durable separators for volumes between milliliter and liter. The LOABeads MagSep series of laboratory magnetic separators have been optimized for use with LOABeads magnetic beads and are ideal for most bioseparation applications.

Product Characteristics

Product name	LOABeads™ MagSep 15/50	LOABeads™ MagSep 500
Product number	3001	4001
Diameter	123 mm	176 mm
Height	119 mm	148 mm
Weight	Approx. 400 g	Approx. 2000 g
Volume	15 and 50 ml	500 ml
Working volume	3-50 ml	250-500 ml
Working range beads*	10 µl-4 ml	1-30 ml
Magnetic separation time	10 seconds	2-5 minutes

* The recommended amount of settled LOABeads

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