Hybrid capture-based target enrichment employs probes to capture target sequences in a NGS library. Hybrid capture sequencing is sensitive and suited for detecting single nucleotide variants, translocations, structural variants, insertions and deletions, and copy number variations. Agilent provides a full suite of hybridization capture-based target enrichment NGS products, including library preparation and target enrichment kits, exomes, catalog and custom probes, software solutions, sample quality control and automation platforms. Whether your research needs a catalog NGS panel or a custom design to include your genes of interest, Agilent has the solutions for you.

Agilent’s amplicon-based technologies for target enrichment and amplification include HaloPlex / HaloPlex^HS and SureMASTR. Both technologies offer target enrichment solutions for NGS with speed, accuracy, and sensitivity.

HaloPlex^HS is a high-sensitivity, amplicon-based targeted sequencing method that incorporates molecular barcodes in the DNA library.

Agilent’s SureMASTR technology is based on multiplex PCR and amplifies multiple genomic targets through a simple PCR experiment.