An Integrated System for In-vitro CRISPR/Cas9 Research

CRISPR/Cas9 is an RNA-guided DNA nuclease associated with Type II bacterial CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) immunity systems. Cas9 (CRISPR associated protein 9) is widely used to induce site-specific double-stranded breaks in DNA for multiple applications. Agilent features an integrated system for in vitro CRISPR/Cas9 research. Agilent’s chemically synthesized gRNAs are the industry standard for high quality, chemically modified single guides. SureGuide gRNA synthesis and control kits allow rapid and simple preparation of guide RNA (gRNAs) to be used separately or bundled with the SureGuide Cas9 system. CRISPR libraries allow researchers to obtain perfect sequence input. The SureVector CRISPR library cloning kits provide reagents for preparation of a lentiviral plasmid library for in vivo expression of gRNA sequences. And the CRISPR Cas9 endonuclease kit provides in vitro cloning of large genes or DNA fragments without the limitations imposed by common restriction enzymes or PCR fidelity.