High Performance Liquid Chromatography System (HPLC) / model "Chromaster"

HPLC for today and tomorrow. The excellent reproducibility made possible by the pump and autosampler and the excellent stability of the column oven and detector are the two performance capabilities supporting data reliability:
The Hitachi reputation for instrument robustness and reliability continues with the Chromaster, which is made using stronger materials and is manufactured with Hitachi's strict quality control standards.

Features :
Modules available : Isocratic Pump which can be upgraded to quaternary by installing gradient kit and degasser. Manual Injector or Autosampler (120 samples). Column Oven with cooling facility. UV, UV-Visible, Fluorescence, RI, Diode Array or MS Detector.

Pump- Improved gradient performance and the excellent flow rate precision : The Chromaster has a new low-pressure mode called High Frequent Mode (HFM), which utilizes a double switching function of proportioning valves. HFM and the Hitachi high speed realtime feedback control system, greatly suppress liquid pulsation for improved reproducibility of gradient and retention times.

Autosampler - Excellent injection volume precision and low carry-over :
The newly adopted high-precision syringe drive unit provides excellent injection volume precision. Hitachi has eliminated the dead volume in the autosampler flow path, which can cause carry-over and adopted a pumping method that washed the needle outer wall. The result is an accurate autosampler with extremely low carry-over.

Column Oven - Pre-heating function and a wide temperature control range : The block-type pre-heating function based on Peltier heating and cooling control delivers excellent symmetric and sharply peak shape. The oven can regulate temperature from 15 degree below ambient temperature to 850C..

Diode Array Detector - Excellent qualitative analysis performance, and extremely low noise and low drift : With a wide wavelength range (190 nm to 900 nm) and excellent resolution (1,024-bit diode array), the Chromaster Diode array detector delivers the world's highest level of high-resolution analyses. With a low noise level , the Diode array detector is capable of supporting high-sensitivity analyses. The adoption of a variable air-volume fan and the provision of a specially designed cover on the spectrometer minimize the influence of temperature fluctuations around the optical system and achieve a further reduction in drift.

UV and UV-VIS Detectors - Two-wavelength, simultaneous high-sensitivity detection of drug impurity : The two-wavelength detection function permits measurements at short data acquisition interval of 400 ms and 800 ms per wavelength, resulting in chromatograms with fine, sharp peak shapes.

Thermostat flow cell : The thermostat flow cell minimizes the influence of ambient temperature changes.
As a result, the baseline of detector is steady and data reliability is improved.

Specifications :
5110/5160 Pump :
1) Solvent delivery system :Dual plunger reciprocating pump system
2) Flow rate : 0.001 to 9.99 ml/min (5110) / 0.001 to 5.00 ml/min (5160)
3) Maximum discharge pressure :40.0 MPa (5110) / 60.0 MPa (5160)
4) Flow rate accuracy :± 2 µL/min or ± 1 % whichever is higher
5) Flow rate precision :SD 0.02 min or RSD 0.075 %, the bigger one
Low pressure gradient unit :
1) Mixture solvent :Max. 4 solvents
2) Mixture system :Solenoid valve open/close control system
3) Mixture ratio :5 to 95 % (Setup/indication by UI pad, 1 % step)
4) Mixture accuracy :± 0.5 % (5 to 95 %)
6-channnel degassing unit :
1) Maximum number of solvents can be processed :6 solvents
2) Maximum flow rate capacity can be processed :5.0 mL/min

Autosampler :
1) Maximum number of samples :120 (1.5 mL), 72 (4 mL) (option), 195 (1 mL) (option)
192 (96-hole micro plate x 2) (option)
768 (384-hole micro plate x 2) (option)
2) Sample injection method :Cut injection, All volume injection, Full loop injection
3) Syringe capacity :175 µL (standard), 70 µL (option)
4) Injection volume reproducibility := 0.2 % RSD (10µl is injected, Cut injection)
= 0.25 % RSD (5µl is injected, Cut injection)
= 0.9 % RSD (1µl is injected, Cut injection)
= 1.0 % RSD (1µl is injected, All volume injection)
= 0.2 % RSD (5µl is injected, Full loop injection)
5) Carry over := 0.003 % (Cut injection)

Column Oven :
1) Temperature control system :Peltier heating/cooling system + air circulating system
2) Temperature setting range :1 to 85 °C (1 °C step)
3) Storage column :300 mm x 3 pcs column
UV Detector :
1) Optical system :Double beam
2) Light source : D2 lamp
3) Measuring wavelength range : 190 to 600 nm
4) Wavelength accuracy :± 1 nm
5) Noise level : 0.5 x 10-5 AU or less
6) Drift :1.0 x 10-4 AU/h or less

UV-VIS Detector :
1) Optical system :Double beam
2) Light source : D2 lamp, W lamp and Hg lamp
3) Measuring wavelength range : 190 to 900 nm
4) Wavelength accuracy :± 1 nm
5) Noise level : 0.5 x 10-5 AU or less
6) Drift :1.0 x 10-4 AU/h or less

Diode Array Detector :
1) Optical system : Single beam ratio photometry
2) Light source : D2 lamp, W lamp and Hg lamp
3) Measuring wavelength range : 190 to 900 nm
4) Wavelength accuracy :± 1 nm
5) Detecting elements : 1024 diodes
6) Noise level : 0.5 x 10-5 AU or less
7) Drift :1.0 x 10-3 AU/h or less

Fluorescence Detector :
1) Optical system :Ratio photometry, intensity of transmitted light is monitored.
2) Light source :150 W Xe lamp, Hg lamp
3) Excitation wavelength setting range :200 to 850 nm, zero order
4) Emission wavelength setting range :250 to 900 nm, zero order
5) Excitation side spectral bandwidth :15 nm
6) Emission side spectral bandwidth :15 nm, 30 nm (Selectable)
7) Wavelength accuracy :± 3 nm
8) Sensitivity : S/N greater than 900 versus a Raman peak of water (Baseline method)

RI Detector :
1) Optical system :Deflection
2) Light source :Tungsten lamp
3) Refractive index :1.0 to 1.75
4) Linearity range : 600 µRIU or more
5) Noise :2.5 nRIU or less (Filled with water, Time constant 3.0 sec.)
6) Drift :0.2 µRIU/h or less (1 mL/ min water)
7) Temperature control range :OFF, 30 to 50 °C

MS Detector :
1) Measurement mass range (m/z) : 20 - 1000
2) Ion source : Electronspray ionization (ESI)

Chromatography Data System :
1) Chromaster system Manager
2) OpenLAB (Agilent)
3) Empower 3 (Waters)

Applications : Chromaster HPLC system can cater to all the chromatography applications (separation and identification) in the fields of food, pharmaceutical, life science and chemistry. For more sensitive analysis, Fluorescence and MS detectors are available. For analysis of Sugars, etc., RI Detector can be used. Following are few examples of its applications -

• Analysis of Organic Acids
• Measurement of water soluble vitamins (3-D measurement using DAD)
• Measurement of natural medicines
• Measurement of phenoxyethanol and parabens
• Peptide mapping
• Analysis of Azo-dyes in carpet industry
• Measurement of Aflatoxins in food
• Measurement of drugs of abuse
• High sensitivity analysis of reduced sugars
• Determination of low concentration of impurities in drugs using MS Detector
• Analysis of pesticides by MS Detector.

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